Sensitive detection of miR-21 and miR-25 in gastric adenocarcinoma patient serum using a SERS sensor based on AuNT and enzyme cleavage strategy.

ABSTRACT: MicroRNA (miRNA) detection has significant application value for early cancer diagnosis. In this study, a surface-enhanced Raman scattering (SERS) sensor was developed for detecting miR-21 and miR-25 in the serum of Gastric adenocarcinoma (GAC) patients. The sensor was constructed using arrays of Au trioctahedral nanoparticles (AuNT) and enzyme cleavage techniques. The AuNT was obtained by self-assembly at the oil-water interface, and the Cy5-labeled miR-21 and 5-FAM-labeled miR-25 complementary single-stranded ssDNA-21 and ssDNA-25 were connected with the AuNT to form the SERS sensor. When miR-21 and miR-25 were present, ssDNA-21 and ssDNA-25 were paired and hybridized to form miR-21-ssDNA-21 and miR-25-ssDNA-25 double strands. Duplex-specific nuclease (DSN) could act on the DNA phosphodi

SUBMITTER: Chen G 

PROVIDER: S-EPMC11808354 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature