On the pixel selection criterion for the calculation of the Pearson's correlation coefficient in fluorescence microscopy.

ABSTRACT: Colocalisation microscopy analysis provides an intuitive and straightforward way of determining if two biomolecules occupy the same diffraction-limited volume. A popular colocalisation coefficient, the Pearson's correlation coefficient (PCC), can be calculated using different pixel selection criteria: PCC_ALL includes all image pixels, PCC_OR only pixels exceeding the intensity thresholds for either one of the detection channels, and PCC_AND only pixels exceeding the intensity thresholds for both detection channels. Our results show that PCC_ALL depends on the foreground to background ratio, producing values influenced by factors unrelated to biomolecular association. PCC_AND focuses on areas with the highest intensities in both channels, w

SUBMITTER: Lopez SG 

PROVIDER: S-EPMC11808421 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature