Gα_o1 and Gα_o1/Gα_o2 deletion differentially affect hippocampal mossy fiber tract anatomy and neuronal morphogenesis.

ABSTRACT: The heterotrimeric G-protein αo subunit is ubiquitously expressed in the CNS as two splice variants Gα_o1 and Gα_o2, regulating various brain functions. Here, we investigated the effect of single Gα_o1, Gα_o2, and double Gα_o1/2 knockout on the postnatal development of the murine mossy fiber tract, a central pathway of the hippocampal connectivity circuit. The size of the hippocampal synaptic termination fields covered by mossy fiber boutons together with various fiber length parameters of the tract was analyzed by immunohistochemical staining of the vesicular Zinc transporter 3 (ZnT3) or Synaptoporin at postnatal days 2, 4, 8, 12, 16, and in the adult. Ultimately, Gα_o1 knockout resulted in a reduced developmental growth of synap

SUBMITTER: Holtje M 

PROVIDER: S-EPMC11808459 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature