Amorphous calcium phosphate-coated surfaces as a model for bone microenvironment in prostate cancer.

ABSTRACT:

Background

Bone metastasis remains one of the biggest challenges in the treatment of prostate cancer, and other solid tumors such as breast, lung, and colon. Modeling a complex microenvironment in-vitro such as the bone niche, requires interrogation of cell-cell interactions, specific extracellular matrix proteins, and a high calcium environment.

Methods

Here, we present a fast and cost-effective system in which commercially available, non-adhesive cell culture vessels are coated with amorphous calcium phosphate (ACP) as a surrogate for bone matrix. We also present modified protocols for subculturing cells and collecting nucleic acids and protein in high-calcium samples.

Results

We find that prostate epithelial cell lines show increased adhesion and proliferation when

SUBMITTER: San Martin R 

PROVIDER: S-EPMC11808503 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature