Chemoselective and laser cleavable probes for <i>in situ</i> protein lipoylation detection by laser desorption/ionization mass spectrometry.

ABSTRACT: Protein lipoylation is a post-translational modification (PTM) of great significance as the lipoylation sites have essential effects on the enzymatic activities of several protein complexes, which affect the biological metabolic pathways and are further related to some diseases, such as cancer and Alzheimer's disease. While proteomic identification of lipoylated proteins has been studied, in situ profiling of protein lipoylation with high sensitivity remains challenging. Herein, we developed a strategy for in situ analysis of protein lipoylation by laser desorption/ionization-mass spectrometry (LDI-MS). In this study, a chemoselective butyraldehyde probe (BAP) was used to label the lipoylated proteins, and then linked with laser cleavable probe modified gold nanoparticles (Au

SUBMITTER: Du Q 

PROVIDER: S-EPMC11808561 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature