Characterization of Sugammadex-Related Isomeric Cyclodextrin Impurities Using Cyclic Ion Mobility High-Resolution Mass Spectrometry.

ABSTRACT: Cyclic ion mobility-mass spectrometry (cIM-MS) is a powerful technique for separating and identifying isomeric mixtures of compounds. When coupled with chromatography, cIM-MS creates a multidimensional separation system, with high resolving power and peak capacity. In this study, we report the cyclic ion mobility separation and high-resolution mass spectrometry identification of four regioisomers of a Sugammadex-related impurity, abbreviated as Di-OH-SGM. Separation using multipass cyclic ion mobility was achieved by selecting the [M + 2Na]²⁺ ion, while other adducts, such as [M + Na]⁺, [M + 2H]²⁺, [M + H + Na]²⁺, and [M - 2H]^2- did not yield isomer separation. Two methods were developed for ion mobility separation of the isomers: a co

SUBMITTER: Szakaly PS 

PROVIDER: S-EPMC11808773 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature