Structural Categorization of Adenine, Guanine, and Xanthine Derivatives Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry with 5-Nitrosalicylic Acid and 1,5-Diaminonaphtalene.

ABSTRACT: In this study, we analyzed purine derivatives using multimatrix variation matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) with α-cyano-4-hydroxycinnamic acid (CHCA), 1,5-diaminonaphtalene (DAN), 5-formylsalicylic acid (FSA), and 5-nitrosalicylic acid (NSA) as matrices. Further, we focused on the abstraction/attachment of hydrogen from/to analytes and detected [M - H]⁺, [M + 2H]^+• and/or [M + 3H]⁺ in MALDI MS spectra of compounds containing nitrogen and/or carbonyl oxygen. Although [M - H]⁺ generation of purine compounds in MALDI MS with conventional matrices was challenging, NSA-MALDI MS effectively yielded the [M - H]⁺species of purine derivatives compared with CHCA, FSA, and DAN, and the [M - H]⁺/[

SUBMITTER: Yamagaki T 

PROVIDER: S-EPMC11808777 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature