Nanopore adaptive sampling to identify the NLR gene family in melon (Cucumis melo L.).

ABSTRACT:

Background

Nanopore adaptive sampling (NAS) offers a promising approach for assessing genetic diversity in targeted genomic regions. Here we designed and validated an experiment to enrich a set of resistance genes in several melon cultivars as a proof of concept.

Results

Using the same reference to guide read acceptance or rejection with NAS, we successfully and accurately reconstructed the 15 regions in two newly assembled ssp. melo genomes and in a third ssp. agrestis cultivar. We obtained fourfold enrichment regardless of the tested samples, but with some variations according to the enriched regions. The accuracy of our assembly was further confirmed by PCR in the agrestis cultivar. We discussed parameters that could influence the enrichment and accuracy of NAS generated

SUBMITTER: Belinchon-Moreno J 

PROVIDER: S-EPMC11808957 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature