Inflammatory responses revealed through HIV infection of microglia-containing cerebral organoids.

ABSTRACT: Cerebral organoids (COs) are valuable tools for studying the intricate interplay between glial cells and neurons in brain development and disease, including HIV-associated neuroinflammation. We developed a novel approach to generate microglia containing COs (CO-iMs) by co-culturing hematopoietic progenitors and inducing pluripotent stem cells. This approach allowed for the differentiation of microglia within the organoids concomitantly with the neuronal progenitors. Compared with conventional COs, CO-iMs were more efficient at generating CD45⁺/CD11b⁺/Iba-1⁺ microglia and presented a physiologically relevant proportion of microglia (~ 7%). CO-iMs presented substantially increased expression of microglial homeostatic and sensome markers as well as markers for

SUBMITTER: Narasipura SD 

PROVIDER: S-EPMC11808982 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature