A cost-effective strategy for identifying Angiostrongylus spp. larvae in Achatina fulica: combined morphological and molecular biology.

ABSTRACT:

Background

The detection of Angiostrongylus spp. larvae in intermediate host snails is a critical epidemiological investigation, essential for the effective control of disease outbreaks. Compared to molecular biological detection methods, lung microscopy, a traditional pathogen morphological detection approach, is susceptible to oversights and exhibits relatively lower sensitivity. However, we posit that lung microscopy offers irreplaceable advantages in the context of large-scale field surveys and can serve as a vital foundation for use in conjunction with other diagnostic technologies.

Methods

In this study, 348 Achatina fulica samples were examined using lung microscopy, PCR, and AcanITS1 qPCR. Statistical analysis was conducted to compare detection rates and sensitivitie

SUBMITTER: Jiang L 

PROVIDER: S-EPMC11809025 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature