Traveling-wave chemotaxis of neutrophil-like HL-60 cells.

ABSTRACT: The question of how changes in chemoattractant concentration translate into the chemotactic response of immune cells serves as a paradigm for the quantitative understanding of how cells perceive and process temporal and spatial information. Here, using a microfluidic approach, we analyzed the migration of neutrophil-like HL-60 cells to a traveling wave of the chemoattractants N-formyl-methionyl-leucyl-phenylalanine (fMLP) and leukotriene B4 (LTB4). We found that under a pulsatile wave that travels at a speed of 95 and 170 µm/min, cells move forward in the front of the wave but slow down and randomly orient at the back due to temporal decrease in the attractant concentration. Under a slower wave, cells reorient and migrate at the back of the wave; thus, cell displacement is canceled out or

SUBMITTER: Ishida M 

PROVIDER: S-EPMC11809305 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature