β-actin function in platelets and red blood cells can be performed by γ-actin and is therefore independent of actin isoform protein sequence.

ABSTRACT: Actin is an essential component of the cytoskeleton in every eukaryotic cell. β-and γ-nonmuscle actin are over 99% identical to each other at the protein level but are encoded by different genes and play distinct roles in vivo. Blood cells, especially red blood cells (RBC), contain almost exclusively β-actin, and it has been generally assumed that this bias is dictated by the unique suitability of β-actin for RBC cytoskeleton function due to its specific amino acid sequence. Here we tested this assumption by analyzing the "β-coded γ-actin" (Actbcg) mouse model, in which the β-actin gene is edited by five-point mutations to produce γ-actin protein. Strikingly, despite lacking β-actin protein, Actbcg mice had no detectable phenotypes in RBCs, and no changes in the RBC shape, integrity, defor

SUBMITTER: Chakravarty D 

PROVIDER: S-EPMC11809312 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature