The Na⁺,K⁺,2Cl^- Cotransporter, Not Aquaporin 1, Sustains Cerebrospinal Fluid Secretion While Controlling Brain K⁺ Homeostasis.

ABSTRACT: Disturbances in the brain fluid balance can lead to life-threatening elevation in intracranial pressure (ICP), which represents a vast clinical challenge. Targeted and efficient pharmaceutical therapy of elevated ICP is not currently available, as the molecular mechanisms governing cerebrospinal fluid (CSF) secretion are largely unresolved. To resolve the quantitative contribution of key choroid plexus transport proteins, this study employs mice with genetic knockout and/or viral choroid plexus-specific knockdown of aquaporin 1 (AQP1) and the Na⁺, K⁺, 2Cl^- cotransporter 1 (NKCC1) for in vivo determinations of CSF dynamics, ex vivo choroid plexus for transporter-mediated clearance of a CSF K⁺ load, and patient CSF for [K⁺] quantificatio

SUBMITTER: Jensen DB 

PROVIDER: S-EPMC11809428 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature