IFlpMosaics enable the multispectral barcoding and high-throughput comparative analysis of mutant and wild-type cells.

ABSTRACT: To understand gene function, it is necessary to compare cells carrying the mutated target gene with normal cells. In most biomedical studies, the cells being compared are in different mutant and control animals and, therefore, do not experience the same epigenetic changes and tissue microenvironment. The experimental induction of genetic mosaics is essential to determine a gene cell-autonomous function and to model the etiology of diseases caused by somatic mutations. Current technologies used to induce genetic mosaics in mice lack either accuracy, throughput or barcoding diversity. Here we present the iFlpMosaics toolkit comprising a large set of new genetic tools and mouse lines that enable recombinase-dependent ratiometric induction and single-cell clonal tracking of multiple fluorescen

SUBMITTER: Garcia-Gonzalez I 

PROVIDER: S-EPMC11810794 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature