Development of new powdery mildew resistant lines in garden pea (<i>Pisum sativum</i> L.) using induced mutagenesis and validation of resistance for the <i>er1</i> and <i>er2</i> gene through molecular markers.

ABSTRACT: Powdery mildew (PM) caused by Erysiphie pisi Syd. is the most devastating disease of pea, affecting fresh pea production as well as the quality of the marketable harvest worldwide. The efforts were made to develop PM-resistant mutants of popular pea varieties "Lincoln" and "Azad P-1" through induced mutations by following gamma irradiation (300, 400, 500, and 600 Gy) and chemical mutagenesis, i.e., ethyl methane sulfonate (EMS) (0.3% and 0.4%). The screening of 13,868 M₂ progenies at Kukumseri (summer season) followed by M₃ generation at Palampur (winter season) resulted in the isolation of six putative PM-resistant mutants. The rigorous evaluation of these progenies under in vivo (field screening) and in vitro (artificial screening under greenhou

SUBMITTER: Sharma A 

PROVIDER: S-EPMC11810882 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature