<i>In silico</i> design of a multi-epitope vaccine against <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i>.

ABSTRACT: The widespread chronic enteritis known as Paratuberculosis (PTB) or Johne's disease (JD) is caused by Mycobacterium avium subspecies paratuberculosis (MAP), posing a significant threat to global public health. Given the challenges associated with PTB or JD, the development and application of vaccines are potentially important for disease control. The aim of this study was to design a multi-epitope vaccine against MAP. A total of 198 MAP genomes were analyzed using pan-genome and reverse vaccinology approaches. B-cell and T-cell epitope analysis was performed on the selected promising cross-protective antigens followed by selection of epitopes with high antigenicity, no allergenicity, and no toxicity for the design of the vaccine. The designed vaccine was evaluated through mol

SUBMITTER: Guo W 

PROVIDER: S-EPMC11810964 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature