Hyperoxia shows duration-dependent effects on the lengths of cell cycle phases in fetal cortical neural stem cells.

ABSTRACT: Fetal neural stem cells (NSCs) physiologically reside under low-oxygen conditions (1%-5% of tissue pO₂), but are often transferred and maintained under atmospheric oxygen levels of 21% pO₂ (hyperoxia) for in vitro investigations. These altered oxygen conditions lead to adaptive changes in NSCs which complicate the interpretation of in vitro data. However, the underlying adaption dynamics remain largely enigmatic. Here we investigated short-term hyperoxia effects (5 days in 3% pO₂ followed by 2 days in 21% pO₂) in comparison to continuous hyperoxia effects (7 days in 21% pO₂) and physioxic control (7 days in 3% pO₂). We utilized cortical NSCs to analyze the cell cycle phases by flow cytometry and cumulative BrdU

SUBMITTER: Lanto J 

PROVIDER: S-EPMC11811091 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature