Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern China.

ABSTRACT:

Background

Bovine hepacivirus (BovHepV) is a recently identified member of the expanding genus Hepacivirus within the family Flaviviridae. However, the genetic diversity, geographical distribution, and host range of the virus remains poorly understood.

Methods

In this study, serum samples from cattle and sheep were obtained in Hulunbuir and pooled to establish RNA libraries, which were then analyzed using transcriptome sequencing. BovHepV-positive samples were confirmed using semi-nested PCR with primers designed based on the obtained viral sequences. Comprehensive bioinformatics analyses were employed to assess sequence similarity, phylogenetic evolution, and recombination of the obtained viral strains.

Results

A total of 988 serum samples from sheep (5

SUBMITTER: Ma J 

PROVIDER: S-EPMC11811627 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature