Allovalent scavenging of activation domains in the transcription factor ANAC013 gears transcriptional regulation.

ABSTRACT: Transcriptional regulation involves interactions between transcription factors, coregulators, and DNA. Intrinsic disorder is a major player in this regulation, but mechanisms driven by disorder remain elusive. Here, we address molecular communication within the stress-regulating Arabidopsis thaliana transcription factor ANAC013. Through high-throughput screening of ANAC013 for transcriptional activation activity, we identify three activation domains within its C-terminal intrinsically disordered region. Two of these overlap with acidic islands and form dynamic interactions with the DNA-binding domain and are released, not only upon binding of target promoter DNA, but also by nonspecific DNA. We show that independently of DNA binding, the RST (RCD--SRO--TAF4) domain of the negative regulato

SUBMITTER: Delaforge E 

PROVIDER: S-EPMC11811731 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature