A distal convoluted tubule-specific isoform of murine SLC41A3 extrudes magnesium.

ABSTRACT:

Background

The distal convoluted tubule (DCT) plays an indispensable role in magnesium (Mg²⁺) reabsorption in the kidney. Yet, the extrusion mechanism of Mg²⁺ has not been identified. The solute carrier 41A3 (SLC41A3) has been suggested to be involved in Mg²⁺ extrusion, but this has never been conclusively demonstrated.

Methods

Using available RNA-sequencing data and real-time quantitative PCR, expression of two alternative Slc41a3 transcripts, encoding isoform (Iso) 1 or 2, were assessed in kidney and isolated DCT tubules. HEK293 or HAP1 cells were transfected with plasmids expressing either of the isoforms, followed by ²⁵Mg²⁺ transport studies. Identification of cis-regulatory elements (CRE) was achieved by combining d

SUBMITTER: Franken GAC 

PROVIDER: S-EPMC11811817 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature