Challenges in observing transcription-translation for bottom-up synthetic biology.

ABSTRACT: Synthetic biology aims to create a viable synthetic cell. However, to achieve this goal, it is essential first to gain a profound understanding of the cellular systems used to build that cell, how to reconstitute those systems in the compartments, and how to track their function. Transcription and translation are two vital cellular systems responsible for the production of RNA and, consequently, proteins, without which the cell would not be able to maintain itself or fulfill its functions. This review discusses in detail how the Protein synthesis Using Recombinant Element (PURE) system and cell lysate are used to reconstitute transcription-translation in vitro. Furthermore, it examines how these systems can be encapsulated in GUVs using the existing methods. It also assesses approaches ava

SUBMITTER: Bogatyr VG 

PROVIDER: S-EPMC11811876 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature