Protein-cell conjugates as artificial surface display for interfacial biocatalysis.

ABSTRACT: Interfacial whole-cell biocatalysis has great potential for advanced chemical synthesis due to its ability to efficiently mediate complex reactions. However, the practical use of this approach is often limited by the fragility of living cells and the difficulty of maintaining enzyme activity under interfacial conditions. Here, we propose an artificial surface display strategy for interfacial biocatalysis by directly coupling sodium caseinate (NaCas) to the surface of E. coli cells. This coupling creates a robust biointerface that provides two main benefits: protecting cells from harsh interfacial environments and enabling the formation of Pickering emulsions for catalysis. The resulting protein-cell conjugates demonstrated thermal stability and strong resistance to organic solvents.

SUBMITTER: Wu X 

PROVIDER: S-EPMC11811893 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature