Enhancing the Bothropic Antivenom through a Reverse Antivenomics Approach.

ABSTRACT: Antivenoms are the only effective treatment for snakebite envenomation and have saved countless lives over more than a century. Despite their value, antivenoms present risks of adverse reactions. Current formulations contain a fraction of nonspecific antibodies and serum proteins. While new promising candidates emerge as the next generation of antivenoms, it remains clear that animal-derived antivenoms will still play a critical role for years to come. In this study, we improved the bothropic antivenom (BAv), by capturing toxin-specific antibodies through affinity chromatography using immobilized Bothrops jararaca venom toxins. This process produced an improved antivenom (iBAv) enriched in neutralizing antibodies and depleted of serum proteins. Proteomic analysis showed that iBAv wa

SUBMITTER: Chiarelli T 

PROVIDER: S-EPMC11812011 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature