Modular plasmid design for autonomous multi-protein expression in Escherichia coli.

ABSTRACT:

Background

Molecular and synthetic biology tools enable the design of new-to-nature biological systems, including genetically engineered microorganisms, recombinant proteins, and novel metabolic pathways. These tools simplify the development of more efficient, manageable, and tailored solutions for specific applications, biocatalysts, or biosensors that are devoid of undesirable characteristics. The key aspect of preparing these biological systems is the availability of appropriate strategies for designing novel genetic circuits. However, there remains a pressing need to explore independent and controllable systems for the co-expression of multiple genes.

Results

In this study, we present the characterisation of a set of bacterial plasmids dedicated to recombinant expression

SUBMITTER: Matera A 

PROVIDER: S-EPMC11812199 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature