Uncovering <i>de novo</i> polyamine biosynthesis in the gut microbiome and its alteration in inflammatory bowel disease.

ABSTRACT: Polyamines are important gut microbial metabolites known to affect host physiology, yet the mechanisms behind their microbial production remain incompletely understood. In this study, we developed a stable isotope-resolved metabolomic (SIRM) approach to track polyamine biosynthesis in the gut microbiome. Viable microbial cells were extracted from fresh human and mouse feces and incubated anaerobically with [U-¹³C]-labeled inulin (tracer). Liquid chromatography-high resolution mass spectrometry analysis revealed distinct ¹³C enrichment profiles for spermidine (SPD) and putrescine (PUT), indicating that the arginine-agmatine-SPD pathway contributes to SPD biosynthesis in addition to the well-known spermidine synthase pathway (PUT aminopropylation). Species differences w

SUBMITTER: Li X 

PROVIDER: S-EPMC11812404 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature