Exploring the Proteomic Landscape and Immunomodulatory Functions of <i>Edwardsiella piscicida</i> Derived Extracellular Vesicles.

ABSTRACT: Extracellular vesicles (EVs) have garnered attention in research for their potential as biochemical transporters and immune modulators, crucial for regulating the host immune system. The present study was conducted to isolate and characterize EVs from Gram negative bacteria Edwardsiella piscicida (EpEVs) and investigate their proteomic profile and immune responses. Isolation of EpEVs was carried out using ultracentrifugation method. Transmission electron microscopy results confirmed the spherical shape of EpEVs. The average size and zeta potential were 85.3 ± 1.8 nm and -8.28 ± 0.41 mV, respectively. EpEVs consisted of 1,487 distinct proteins. Subcellular localization analysis revealed that "cell" and "cell part" were the most predominant areas for protei

SUBMITTER: Dias MKHM 

PROVIDER: S-EPMC11813346 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature