Signaling network involved in the GPC3-induced inhibition of breast cancer progression: role of canonical Wnt pathway.

ABSTRACT:

Purpose

We have shown that GPC3 overexpression in breast cancer cells inhibits in vivo tumor progression, by acting as a metastatic suppressor. GPC3-overexpressing cells are less clonogenic, viable and motile, while their homotypic adhesion is increased. We have presented evidences indicating that GPC3 inhibits canonical Wnt and Akt pathways, while non-canonical Wnt and p38MAPK cascades are activated. In this study, we aimed to investigate whether GPC3-induced Wnt signaling inhibition modulates breast cancer cell properties as well as to describe the interactions among pathways modulated by GPC3.

Methods

Fluorescence microscopy, qRT-PCR microarray, gene reporter assay and Western blotting were performed to determine gene expression levels, signaling pathway activities and mo

SUBMITTER: Fernandez D 

PROVIDER: S-EPMC11813400 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature