TIS21^/BTG2 inhibits breast cancer growth and progression by differential regulation of mTORc1 and mTORc2-AKT1-NFAT1-PHLPP2 signaling axis.

ABSTRACT:

Purpose

It has been reported that PI3K/AKT pathway is altered in various cancers and AKT isoforms specifically regulate cell growth and metastasis of cancer cells; AKT1, but not AKT2, reduces invasion of cancer cells but maintains cancer growth. We propose here a novel mechanism of the tumor suppresser, TIS21^/BTG2, that inhibits both growth and invasion of triple negative breast cancer cells via AKT1 activation by differential regulation of mTORc1 and mTORc2 activity.

Methods

Transduction of adenovirus carrying TIS21^/BTG2 gene and transfection of short interfering RNAs were employed to regulate TIS21^/BTG2 gene expression in various cell lines. Treatment of mTOR inhibitors and mTOR kinase assays can evaluate the role of mTORc in the regulation

SUBMITTER: Sundaramoorthy S 

PROVIDER: S-EPMC11813427 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature