Identification of aberrantly expressed F-box proteins in squamous-cell lung carcinoma.

ABSTRACT:

Purpose

F-box proteins, as components of the Skp1-Cullin 1-F-box protein (SCF) E3 ubiquitin ligase, can specifically bind to substrates and regulate multiple tumor behaviors. However, the role of F-box proteins in squamous-cell lung carcinoma (SqCLC) has not been established.

Methods

We identified the differentially expressed F-box protein-encoding genes in SqCLC by analyzing data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Prognosis data were evaluated using the Kaplan-Meier (KM) plotter website. The FBXO5 and FBXO45 mRNA levels were analyzed by real time RT-PCR. The impact of the inhibition of these genes with si-RNA on apoptosis and migration was also investigated.

Results

The FBXO45 and FBXO5 genes were significantly up-regulat

SUBMITTER: Wang K 

PROVIDER: S-EPMC11813518 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature