N-Doped carbon quantum dot-based ratiometric fluorescent nanosensor platforms for detection of gastric cancer-associated Helicobacter pylori genes.

ABSTRACT: Carbon quantum dot (CQD)-based fluorescent nanosensor platforms were developed using gastric cancer-associated Heliobacter pylori (H. pylori) genes. N-doped CQDs were synthesized using two different organic acids (citric acid and malic acid) and ethylenediamine by the microwave method. The photophysical and photochemical properties of the synthesized CQDs were investigated by ultraviolet-visible, fluorescence, and Fourier-transform infrared spectra. The surface of the synthesized N-doped CQDs was conjugated with single-stranded DNA (ssDNA), which is specific for gastric cancer. Ethidium bromide, a selective dye, shows enhanced fluorescence intensity upon intercalating with DNA. In the blue-emissive CQD-ssDNA nanoprobe system, the fluorescence intensity was quenched by ethidium bromide due

SUBMITTER: Ozturk D 

PROVIDER: S-EPMC11813823 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature