Induction of somatic embryogenesis and cryopreservation of <i>Abies pinsapo</i> Boiss.

ABSTRACT: Abies pinsapo is an endangered species, endemic to southern Spain. Somatic embryogenesis and cryopreservation constitute important biotechnological tools, which can be used in order to improve the management and conservation of threatened species. The objective of this work was to develop somatic embryogenesis and cryopreservation protocols for A. pinsapo. Somatic embryogenesis was induced from mature zygotic embryos of A. pinsapo cultured on solid MS medium with macroelements at half-strength and supplemented with 20 g L^-1 sucrose and 5 mg L^-1 6-benzylaminopurine (BA). Embryogenic cultures successfully proliferated on solid medium with the same formulation supplemented with 20 g L^-1 sucrose, 500 mg L^-1 L-glutamine, 1 g L

SUBMITTER: Cabero-Moreno L 

PROVIDER: S-EPMC11813934 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature