Assessment of the influence of 660 and 808-nm PBM treatments on mitochondrial oxygen consumption of MG-63 osteoblast: a 3D cell culture study.

ABSTRACT: This study explores the dose-dependent effects of 660-nm and 808-nm photobiomodulation (PBM) on mitochondrial oxygen respiration rate activity in MG-63 osteoblast cells using an innovative 3D in vitro spheroid model. MG-63 osteoblast cells were grown to 80% confluence and seeded in fish gelatin hydrogel (LunaGel™) to form 3D spheroids within 3-7 days. Spheroids were seeded on Seahorse microplates and incubated in a LunacrossLinker™ (visible light crosslinking system) for 2 min to give hydrogel a mid-stiffness of 3.5 kPa. Cells were exposed to PBM either 660-nm or 808-nm at panel setting of 5 J/cm² and 15 J/cm² and then assessed immediate (15 min before analysing) and 24 h time points. Mitochondrial activity was determined using an XFe96 Seahorse analyzer. Data distrib

SUBMITTER: Sleep S 

PROVIDER: S-EPMC11813955 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature