Toward the development of a specific non-enzymatic amperometric sensor for determining uric acid in fermentation samples.

ABSTRACT: The development is proposed of a specific non-enzymatic amperometric sensor based on electrodeposited copper nanoparticles (Cu-NPs) for the determination of uric acid (UA) in fermentation samples. Through optimization of the Cu-NPs-containing sensing layer, it was demonstrated that copper(II)-induced oxidation (catalytic effect) in the presence of molecular oxygen is more effective for determining UA than the adsorption of UA on Cu and Cu-oxide surfaces. More importantly, simply changing the sensing layer's surface chemistry by increasing the defect Cu_xO_y on the surface of Cu-NPs after heating at 70 °C for only 20 min significantly improved the specificity of UA determination in both model and real fermentation samples (viz. supernatants of S. cerevisiae and E. coli).

SUBMITTER: Butyrskaya EV 

PROVIDER: S-EPMC11814019 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature