Porcine myogenesis in cloned wildtype and MYF5/MYOD/MYF6-null porcine embryo.

ABSTRACT: The pig is an important animal model increasingly used for biomedical research, particularly in transplantation strategies involving xenotransplantation or the development of human organs in pig for exotransplantation. Pigs, however, are less characterized than other animal models. In this study, we produced wildtype (WT) pig embryos via somatic cell nuclear transfer (SCNT) technology and compared them to skeletal muscle null embryos (lacking MYF5/MYOD/MYF6) at embryonic day 41, 62, and 90, critical stages of porcine myogenesis. Magnetic resonance imaging (MRI) and histological analyses revealed progressive development of skeletal muscle in WT embryos but not in null embryos whereas development of viscera progressed equally in both groups. Molecular analyses highlighted dynamic changes in

SUBMITTER: Choe YH 

PROVIDER: S-EPMC11814088 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature