Rapid antimicrobial susceptibility testing using carbon screen printed electrodes in a microfluidic device.

ABSTRACT: The development of rapid, sensitive, and affordable antimicrobial susceptibility testing (AST) is essential for controlling antibiotic overuse, thereby creating a critical checkpoint for the emerging antimicrobial resistance threat. Here, we introduce a novel method of electrochemical monitoring of bacterial growth in a diluted low-conductivity nutrient medium for rapid susceptibility testing using impedance spectroscopy. The method works on the change in charge transfer resistance exhibited by bacteria in response to antibiotics. The proposed Electrochemical Microfluidic device (ε-µD) employs low-cost carbon screen-printed electrodes and uses a simple microfluidic geometry. We explored the utilisation of a diluted nutrient medium as an electrolyte since it provides a higher charge transfe

SUBMITTER: Gopalakrishnan S 

PROVIDER: S-EPMC11814112 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature