Assembly and network of Rhei Radix et Rhizoma surface microbiome shaped by processing methods and sampling locations.

ABSTRACT:

Objective

Rhei Radix et Rhizoma has five types of products, namely, raw rhubarb (RR), wine rhubarb (WR), vinegar rhubarb (VR), cooked rhubarb (CR), and rhubarb charcoal (RC). However, Rhei Radix et Rhizoma is easily contaminated with fungi and mycotoxins if not harvested or processed properly. Here, we intend to analyze how microbiome assemblies and co-occurrence patterns are influenced by sampling locations and processing methods.

Methods

High-throughput sequencing and internal transcribed spacer 2 (ITS2) were carried out to study the diversities (α- and β-diversity), composition (dominant taxa and potential biomarkers), and network complexitity of surface fungi on RR, WR, VR, CR, and RC collected from Gansu and Sichuan provinces, China.

Resul

SUBMITTER: Wei G 

PROVIDER: S-EPMC11814265 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature