The spatiotemporal transcriptional profiling of murine brain during cerebral malaria progression and after artemisinin treatment.

ABSTRACT: Cerebral malaria (CM) is a severe encephalopathy caused by Plasmodium parasite infection, resulting in thousands of annual deaths and neuro-cognitive sequelae even after anti-malarial drugs treatment. Despite efforts to dissect the mechanism, the cellular transcriptomic reprogramming within the spatial context remains elusive. Here, we constructed single-cell and spatial transcriptome atlases of experimental CM (ECM) male murine brain tissues with or without artesunate (ART) treatment. We identified activated inflammatory endothelial cells during ECM, characterized by a disrupted blood-brain barrier, increased antigen presentation, and leukocyte adhesion. We also observed that inflammatory microglia enhance antigen presentation pathway such as MHC-I to CD8⁺ cytotoxic T cells. Th

SUBMITTER: Chen J 

PROVIDER: S-EPMC11814382 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature