Hyperosmotic Stress Promotes the Nuclear Translocation of TFEB in Tubular Epithelial Cells Depending on Intracellular Ca²⁺ Signals via TRPML Channels.

ABSTRACT:

Purpose

We previously demonstrated that hyperosmotic stress, which acts as mechanical stress, induces autophagy of tubular epithelial cells. This study aims to elucidate the molecular mechanisms of hyperosmolarity-induced autophagy. The research question addresses how hyperosmotic stress activates autophagy through transcription factor EB (TFEB) and Ca²⁺ signaling pathways, contributing to understanding cellular responses to mechanical stress.

Methods

NRK-52E normal rat kidney cells were subjected to hyperosmotic stress using mannitol-containing medium. Fluorescence microscopy was utilized to observe TFEB nuclear translocation, a crucial event in autophagy regulation. An intracellular Ca²⁺ chelator, BAPTA-AM, and a calcineurin inhibitor were used to dis

SUBMITTER: Miyano T 

PROVIDER: S-EPMC11814421 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature