Discovery of conserved peptide-MHC epitopes for directly alloreactive CD8⁺ T cells.

ABSTRACT: Mass Spectrometry allied with in-vivo generation of activated alloreactive T cell populations and tetramer screening facilitates the identification of endogenous peptides that are directly recognised in complex with allogeneic Major Histocompatibility class I (MHC I) molecules by alloreactive CD8⁺ T cells. We had previously used this approach for the discovery of immunogenic self-peptides presented by the allomorph H-2K^b (K^b). In this study, we identified 22 highly immunogenic self-peptides presented by H-2K^d (K^d). Peptide abundance across skin, spleen and liver samples (estimated as the product of the spectral intensity obtained for these samples) was the principal factor influencing recognition of peptide-K^d epitope

SUBMITTER: Hill AE 

PROVIDER: S-EPMC11814428 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature