Droplet Digital PCR Provides Highly Sensitive and Accurate Opsin Gene SNP Detection From Wild Primate Fecal Samples.

ABSTRACT: Evaluating field-sourced samples with poor-quality and low-quantity DNA, like animal feces, presents significant challenges in the field of molecular biology. Nonetheless, recent innovations in PCR technology are promoted as effective tools to overcome many of these issues. Here, we evaluate the efficiency of droplet digital PCR (ddPCR) as a method for color vision assessment from feces of white-faced capuchins (Cebus imitator) and report frequencies of alleles and genotypes in a wild population. The sex-linked color vision polymorphism of monkeys in the Americas is driven by single nucleotide polymorphisms (SNPs) in opsin genes at up to three tuning sites. DNA was extracted from fecal samples collected from 211 wild capuchins (53.1% males) in Sector Santa Rosa, Costa Rica: 56 were

SUBMITTER: Fernandes AG 

PROVIDER: S-EPMC11814474 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature