Application of 2D polymerase chain reaction for single-tube detection of high-risk human papillomaviruses.

ABSTRACT:

Purpose

The persistent infection of high-risk HPV (HR-HPV) is intricately linked to the onset and progression of cervical cancer. This research endeavored to develop a high-throughput 2D PCR method for closed-tube genotyping of 11 HR-HPVs.

Methods

Base-quenched probes were specifically designed for FAM, VIC, and CY5 channels. The 2D PCR system underwent optimization, with its detection performance assessed in terms of specificity and sensitivity. Plasmid mixtures was used to simulate multiple infections of HPV, providing preliminary insights into the detection efficacy and throughput of the 2D PCR technology. Ultimately, the detection capability of this method was assessed using clinical samples.

Results

The sequenced tags, when paired with primers, could generate Tm

SUBMITTER: Wang W 

PROVIDER: S-EPMC11815664 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature