DEAPR: Differential Expression and Pathway Ranking Tool Demonstrates <i>NRAS^G12V</i> and <i>NRAS^G12D</i> Mutations Have Differing Effects in THP-1 Cells.

ABSTRACT: Background/Objectives: NRAS mutations are found in approximately 10% of patients with acute myeloid leukemia (AML), with nearly half of those occurring at codon 12, but little is known about how differing G12 mutants affect cancer cell activity. Methods: A novel bioinformatic technique, differential expression and pathway ranking (DEAPR), was used to identify the most prominent changes in terms of both individual genes and associated pathways when comparing AML THP-1 cells containing an NRAS^G12D mutation with B11 cells, which are THP-1-derived cells with the NRAS^G12D allele removed and a dox-inducible NRAS^G12V allele introduced. Results: In total, 1456 differentially expressed (DE) protein-coding genes were u

SUBMITTER: Rathe SK 

PROVIDER: S-EPMC11816133 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature