Identification of a TonB-Dependent Siderophore Receptor as a Novel Anti-Biofilm Target and Virtual Screening for Its Inhibitor in Pseudomonas fluorescens PF08.

ABSTRACT: Pseudomonas fluorescens is a vital food spoilage bacterium that commonly spoils foods in the biofilm state. Uncovering the targets responsible for biofilm formation and disrupting their function is a promising way to control bacterial biofilms and food spoilage. In this work, using the combination of qRT-PCR and construction of the gene deletion strain, Δtdsr, TonB-dependent siderophore receptor D7M10_RS23410 was, for the first time, proven to play an essential part in the biofilm development of P. fluorescens. By utilizing structure-based virtual screening technology, a natural compound, adenosine monophosphate (AMP), with the highest binding activity to D7M10_RS23410, was obtained as an effective biofilm inhibitor. AMP significantly decreased the cell autoaggregation

SUBMITTER: Shen T 

PROVIDER: S-EPMC11816823 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature