Genetic and Physiological Characterization of the Pentose Phosphate Pathway in the Yeast <i>Kluyveromyces lactis</i>.

ABSTRACT: The pentose phosphate pathway (PPP) is essential for human health and provides, amongst others, the reduction power to cope with oxidative stress. In contrast to the model baker's yeast, the PPP also contributes to a large extent to glucose metabolism in the milk yeast Kluyveromyces lactis. Yet, the physiological consequences of mutations in genes encoding PPP enzymes in K. lactis have been addressed for only a few. We here embarked on a systematic study of such mutants, deleting ZWF1, SOL4, GND1, RKI1, RPE1, TKL1, TAL1, and SHB17. Interestingly, GND1, RKI1, and TKL1 were found to be essential under standard growth conditions. Epistasis analyses revealed that a lack of Zwf1 rescued the lethality of t

SUBMITTER: Bertels LK 

PROVIDER: S-EPMC11817168 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature