Ligand-Independent Spontaneous Activation of Purinergic P2Y₆ Receptor Under Cell Culture Soft Substrate.

ABSTRACT: G protein-coupled receptors (GPCRs) exist in the conformational equilibrium between inactive state and active state, where the proportion of active state in the absence of a ligand determines the basal activity of GPCRs. Although many GPCRs have different basal activity, it is still unclear whether physiological stresses such as substrate stiffness affect the basal activity of GPCRs. In this study, we identified that purinergic P2Y₆ receptor (P2Y₆R) induced spontaneous Ca²⁺ oscillation without a nucleotide ligand when cells were cultured in a silicon chamber. This P2Y₆R-dependent Ca²⁺ oscillation was absent in cells cultured in glass dishes. Coating substrates, including collagen, laminin, and fibronectin, did not affect the P2Y_6<

SUBMITTER: Nishimura A 

PROVIDER: S-EPMC11817550 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature