Exploiting Mixed Waste Office Paper Containing Lignocellulosic Fibers for Alternatively Producing High-Value Succinic Acid by Metabolically Engineered <i>Escherichia coli</i> KJ122.

ABSTRACT: Succinic acid is applied in many chemical industries in which it can be produced through microbial fermentation using lignocellulosic biomasses. Mixed-waste office paper (MWOP) containing lignocellulosic fibers is enormously generated globally. MWOP is recycled into toilet paper and cardboard, but the recovery process is costly. The reuse of MWOP to alternatively produce succinic acid is highly attractive. In this study, pretreatment of MWOPs with 1% (v/v) H₂SO₄ at 121 °C for 20 min was found to be optimal. The optimal conditions for the enzymatic hydrolysis of H₂SO₄-pretreated MWOP (AP-MWOP) were at 50 °C, with cellulase loading at 80 PCU/g AP-MWOP. This resulted in the highest glucose (22.46 ± 0.15 g/L) and xylose (5.11 ± 0.32 g/L

SUBMITTER: Congthai W 

PROVIDER: S-EPMC11817568 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature