Rapid Natural Killer Cell Gene Responses, Generated by TLR Ligand-Induced Trained Immunity, Provide Protection to Bacterial Infection in <i>rag1^-/-</i> Mutant Zebrafish (<i>Danio rerio</i>).

ABSTRACT: T and B cell-deficient rag1^-/- mutant zebrafish develop protective immunity mediated by trained immunity. In mammals, trained immune responses can be induced by Toll-like receptor (TLR) ligands. This study evaluated protective trained immunity in rag1^-/- zebrafish through exposure to TLR ligands (beta glucan, R848, poly I:C), RE33^® (a live-attenuated Edwardsiella ictaluri vaccine), or combinations thereof, followed by wild-type E. ictaluri challenge one month later. Survival analyses revealed that all TLR ligands and vaccine treatments provided significantly higher protection than the control, with beta glucan inducing significantly greater protection than RE33^®, while R848 and poly I:C were equivalent to the vaccine.

SUBMITTER: Muire PJ 

PROVIDER: S-EPMC11818001 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature