Optimal Chimeric Antigen Receptor (CAR)-mRNA for Transient CAR T Cell Generation.

ABSTRACT: Genetically modified T lymphocytes expressing chimeric antigen receptors (CARs) are becoming increasingly important in the treatment of hematologic malignancies and are also intensively being investigated for other diseases such as autoimmune disorders and HIV. Current CAR T cell therapies predominantly use viral transduction methods which, despite their efficacy, raise safety concerns related to genomic integration and potentially associated malignancies as well as labor- and cost-intensive manufacturing. Therefore, non-viral gene transfer methods, especially mRNA-based approaches, have attracted research interest due to their transient modification and enhanced safety profile. In this study, the optimization of CAR-mRNA for T cell applications is investigated, focusing on the impact of m

SUBMITTER: Kitte R 

PROVIDER: S-EPMC11818003 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature