Comparison of Recombination Rate, Reference Bias, and Unique Pangenomic Haplotypes in <i>Cannabis sativa</i> Using Seven De Novo Genome Assemblies.

ABSTRACT: Genomic characterization of Cannabis sativa has accelerated rapidly in the last decade as sequencing costs have decreased and public and private interest in the species has increased. Here, we present seven new chromosome-level haplotype-phased genomes of C. sativa. All of these genotypes were alive at the time of publication, and several have numerous years of associated phenotype data. We performed a k-mer-based pangenome analysis to contextualize these assemblies within over 200 existing assemblies. This allowed us to identify unique haplotypes and genomic diversity among Cannabis sativa genotypes. We leveraged linkage maps constructed from F₂ progeny of two of the assembled genotypes to characterize the recombination rate across the genome showing

SUBMITTER: Stack GM 

PROVIDER: S-EPMC11818205 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature