Modulation of β-Catenin promotes WNT expression in macrophages and mitigates intestinal injury.

ABSTRACT:

Background

Macrophages are the major source of WNT ligands. However, the regulation of WNT expression in macrophages has not been studied. In the present study, we have discovered that activation of canonical β-Catenin signaling suppresses WNT expression in macrophages. EVs from these pre-conditioned macrophages promoted intestinal stem cell regeneration and mitigated intestinal injury.

Method

ChIP-seq analysis and validation studies using recombinant DNA construct expressing Luciferase reporter under WNT promoter (e.g. WNT5a and WNT9b) were conducted to demonstrate the involvement of β-Catenin in the transcriptional regulation of WNT expression. The regulatory role of β-Catenin in WNT expression in macrophages was examined by treating these cells with a Tankyrase inhibitor.

SUBMITTER: Chugh RM 

PROVIDER: S-EPMC11818365 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature